Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution
نویسندگان
چکیده
منابع مشابه
Regulation of the integrase and cassette promoters of the class 1 integron by nucleoid-associated proteins.
The integrase IntI1 catalyses recombination of antibiotic-resistance gene cassettes in the integron, a widely found bacterial mobile element active in spreading antibiotic multi-resistance. We have previously shown that resistance cassette recombination rate and specificity depend on the amount of intracellular integrase. Here, we used in vivo and in vitro methods to examine convergent expressi...
متن کاملThe Microbiota and Abundance of the Class 1 Integron-Integrase Gene in Tropical Sewage Treatment Plant Influent and Activated Sludge
Bacteria are assumed to efficiently remove organic pollutants from sewage in sewage treatment plants, where antibiotic-resistance genes can move between species via mobile genetic elements known as integrons. Nevertheless, few studies have addressed bacterial diversity and class 1 integron abundance in tropical sewage. Here, we describe the extant microbiota, using V6 tag sequencing, and quanti...
متن کاملFunctional Speed Reserve as a Proxy for the Anaerobic Speed Reserve Using the Critical Speed Concept
Background: Although maximal sprint speed (MSS) and the anaerobic speed reserve (ASR) provides valuable information about the speed profile of athletes, these parameters fall short of providing important information about sub-maximal metabolic thresholds. The only field test that can offer an estimate of a sub-maximal metabolic threshold is the 3-minute all-out test for running (3MT) which deli...
متن کاملComparative study of class 1 integron and Vibrio cholerae superintegron integrase activities.
Superintegrons (SIs) and multiresistant integrons (MRIs) have two main structural differences: (i) the SI platform is sedentary, while the MRI platform is commonly associated with mobile DNA elements and (ii) the recombination sites (attC) of SI gene cassette clusters are highly homogeneous, while those of MRI cassette arrays are highly variable in length and sequence. In order to determine if ...
متن کاملNovel streptomycin and spectinomycin resistance gene as a gene cassette within a class 1 integron isolated from Escherichia coli.
The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance gene dfr7 in a class 1 integron. The integron was located on a plasmid and was identified ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The ISME Journal
سال: 2014
ISSN: 1751-7362,1751-7370
DOI: 10.1038/ismej.2014.226